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daniovision recording chamber  (Noldus Information Technology)

 
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    Structured Review

    Noldus Information Technology daniovision recording chamber
    A . Schematic of the FishNAP assay, with up to seven larvae per well. Overall activity is monitored using <t>Daniovision</t> for 1 hour after loperamide exposure; increased BBB permeability leads to reduced locomotor activity. B . Leaky 3 dpf larvae are significantly sedated compared to 5 and 7 dpf fish, as previously reported (O’Brown, 2019). C . spock1 mutants show significantly reduced activity compared to wild-type (WT) controls at 7 dpf using FishNAP. D . Workflow schematic for assessing BBB permeability in cldn5a crispant fish. E–H . Tracer leakage assays using 1 kDa NHS ester (E, turquoise) and 10 kDa Dextran (F, gold) reveal significantly increased 1 kDa NHS tracer permeability outside of the kdrl:mCherry + vasculature (magenta) in cldn5a crispants vs. uninjected controls (G), but no significant change in 10 kDa Dextran permeability (H). I . FishNAP also detects increased BBB permeability in cldn5a crispants relative to uninjected controls. The slowest fish (red outlines) show greater than 95% cldn5a knockdown. ** p<0.01, *** p<0.001, **** p<0.0001 by one-way ANOVA (B and C) and Welches t-test (G-I). Scale bar represents 50 µm.
    Daniovision Recording Chamber, supplied by Noldus Information Technology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/daniovision+recording+chamber/chamber+daniovision+observation/bio_rxiv__64898__2026__03__18__712473-122-7-10
    Average 86 stars, based on 1 article reviews
    daniovision recording chamber - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "In vivo discovery of blood-brain barrier opening small molecules with FishNAP"

    Article Title: In vivo discovery of blood-brain barrier opening small molecules with FishNAP

    Journal: bioRxiv

    doi: 10.64898/2026.03.18.712473

    A . Schematic of the FishNAP assay, with up to seven larvae per well. Overall activity is monitored using Daniovision for 1 hour after loperamide exposure; increased BBB permeability leads to reduced locomotor activity. B . Leaky 3 dpf larvae are significantly sedated compared to 5 and 7 dpf fish, as previously reported (O’Brown, 2019). C . spock1 mutants show significantly reduced activity compared to wild-type (WT) controls at 7 dpf using FishNAP. D . Workflow schematic for assessing BBB permeability in cldn5a crispant fish. E–H . Tracer leakage assays using 1 kDa NHS ester (E, turquoise) and 10 kDa Dextran (F, gold) reveal significantly increased 1 kDa NHS tracer permeability outside of the kdrl:mCherry + vasculature (magenta) in cldn5a crispants vs. uninjected controls (G), but no significant change in 10 kDa Dextran permeability (H). I . FishNAP also detects increased BBB permeability in cldn5a crispants relative to uninjected controls. The slowest fish (red outlines) show greater than 95% cldn5a knockdown. ** p<0.01, *** p<0.001, **** p<0.0001 by one-way ANOVA (B and C) and Welches t-test (G-I). Scale bar represents 50 µm.
    Figure Legend Snippet: A . Schematic of the FishNAP assay, with up to seven larvae per well. Overall activity is monitored using Daniovision for 1 hour after loperamide exposure; increased BBB permeability leads to reduced locomotor activity. B . Leaky 3 dpf larvae are significantly sedated compared to 5 and 7 dpf fish, as previously reported (O’Brown, 2019). C . spock1 mutants show significantly reduced activity compared to wild-type (WT) controls at 7 dpf using FishNAP. D . Workflow schematic for assessing BBB permeability in cldn5a crispant fish. E–H . Tracer leakage assays using 1 kDa NHS ester (E, turquoise) and 10 kDa Dextran (F, gold) reveal significantly increased 1 kDa NHS tracer permeability outside of the kdrl:mCherry + vasculature (magenta) in cldn5a crispants vs. uninjected controls (G), but no significant change in 10 kDa Dextran permeability (H). I . FishNAP also detects increased BBB permeability in cldn5a crispants relative to uninjected controls. The slowest fish (red outlines) show greater than 95% cldn5a knockdown. ** p<0.01, *** p<0.001, **** p<0.0001 by one-way ANOVA (B and C) and Welches t-test (G-I). Scale bar represents 50 µm.

    Techniques Used: Activity Assay, Permeability, Knockdown

    Related Articles

    Software:

    Article Title: MED27 function is essential for cerebellar development and motor behaviour
    Article Snippet: .. Data were analyzed by quantifying the total distance swam by DanioVision recording chamber (Noldus) and data were collected using the Ethovision XT 12 software (Noldus). .. H&E analysis of med27 F0 KO zebrafish model Zebrafish larvae at 3 and 5 dpf were fixed in 4% paraformaldehyde (PFA, Thermo Scientific) and embedded in paraffin.

    Activity Assay:

    Article Title: A zebrafish-based in vivo model of Zika virus infection unveils alterations of the glutamatergic neuronal development and NS4A as a key viral determinant of neuropathogenesis.
    Article Snippet: .. Following 30 minutes of habituation in the DanioVision recording chamber (Noldus), locomotor activity upon light stimulation was monitored for two hours. .. Swimming distance (in mm) and immobility times (in seconds) analysis were performed using the EthoVision XT12 software (Noldus).

    Article Title: A zebrafish-based in vivo model of Zika virus infection unveils alterations of the glutamatergic neuronal development and NS4A as a key viral determinant of neuropathogenesis
    Article Snippet: .. Following 30 minutes of habituation in the DanioVision recording chamber (Noldus), locomotor activity upon light stimulation was monitored for two hours. .. Swimming distance (in mm) and immobility times (in seconds) analysis were performed using the EthoVision XT12 software (Noldus).

    other:

    Article Title: Novel loss-of-function variants expand ABCC9 -related intellectual disability and myopathy syndrome
    Article Snippet: We monitored the swimming behaviour of 7 days post-fertilization (dpf) larvae separated into single wells of a 96-well plate containing 200 μl of E3 media and habituated in the Daniovision (Noldus) recording chamber for 1 h before the start of the experiment.

    Article Title: Targeting MMP-9 activation after early-life seizures reduces seizure susceptibility and memory deficits in a larval zebrafish model.
    Article Snippet: Specifically, individual naïve 21 dpf zebrafish were pipetted into a well of a 12-well plate in the DanioVision recording chamber (Noldus).



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    Noldus Information Technology daniovision recording chamber
    A . Schematic of the FishNAP assay, with up to seven larvae per well. Overall activity is monitored using <t>Daniovision</t> for 1 hour after loperamide exposure; increased BBB permeability leads to reduced locomotor activity. B . Leaky 3 dpf larvae are significantly sedated compared to 5 and 7 dpf fish, as previously reported (O’Brown, 2019). C . spock1 mutants show significantly reduced activity compared to wild-type (WT) controls at 7 dpf using FishNAP. D . Workflow schematic for assessing BBB permeability in cldn5a crispant fish. E–H . Tracer leakage assays using 1 kDa NHS ester (E, turquoise) and 10 kDa Dextran (F, gold) reveal significantly increased 1 kDa NHS tracer permeability outside of the kdrl:mCherry + vasculature (magenta) in cldn5a crispants vs. uninjected controls (G), but no significant change in 10 kDa Dextran permeability (H). I . FishNAP also detects increased BBB permeability in cldn5a crispants relative to uninjected controls. The slowest fish (red outlines) show greater than 95% cldn5a knockdown. ** p<0.01, *** p<0.001, **** p<0.0001 by one-way ANOVA (B and C) and Welches t-test (G-I). Scale bar represents 50 µm.
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    A . Schematic of the FishNAP assay, with up to seven larvae per well. Overall activity is monitored using <t>Daniovision</t> for 1 hour after loperamide exposure; increased BBB permeability leads to reduced locomotor activity. B . Leaky 3 dpf larvae are significantly sedated compared to 5 and 7 dpf fish, as previously reported (O’Brown, 2019). C . spock1 mutants show significantly reduced activity compared to wild-type (WT) controls at 7 dpf using FishNAP. D . Workflow schematic for assessing BBB permeability in cldn5a crispant fish. E–H . Tracer leakage assays using 1 kDa NHS ester (E, turquoise) and 10 kDa Dextran (F, gold) reveal significantly increased 1 kDa NHS tracer permeability outside of the kdrl:mCherry + vasculature (magenta) in cldn5a crispants vs. uninjected controls (G), but no significant change in 10 kDa Dextran permeability (H). I . FishNAP also detects increased BBB permeability in cldn5a crispants relative to uninjected controls. The slowest fish (red outlines) show greater than 95% cldn5a knockdown. ** p<0.01, *** p<0.001, **** p<0.0001 by one-way ANOVA (B and C) and Welches t-test (G-I). Scale bar represents 50 µm.
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    A . Schematic of the FishNAP assay, with up to seven larvae per well. Overall activity is monitored using <t>Daniovision</t> for 1 hour after loperamide exposure; increased BBB permeability leads to reduced locomotor activity. B . Leaky 3 dpf larvae are significantly sedated compared to 5 and 7 dpf fish, as previously reported (O’Brown, 2019). C . spock1 mutants show significantly reduced activity compared to wild-type (WT) controls at 7 dpf using FishNAP. D . Workflow schematic for assessing BBB permeability in cldn5a crispant fish. E–H . Tracer leakage assays using 1 kDa NHS ester (E, turquoise) and 10 kDa Dextran (F, gold) reveal significantly increased 1 kDa NHS tracer permeability outside of the kdrl:mCherry + vasculature (magenta) in cldn5a crispants vs. uninjected controls (G), but no significant change in 10 kDa Dextran permeability (H). I . FishNAP also detects increased BBB permeability in cldn5a crispants relative to uninjected controls. The slowest fish (red outlines) show greater than 95% cldn5a knockdown. ** p<0.01, *** p<0.001, **** p<0.0001 by one-way ANOVA (B and C) and Welches t-test (G-I). Scale bar represents 50 µm.
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    A . Schematic of the FishNAP assay, with up to seven larvae per well. Overall activity is monitored using <t>Daniovision</t> for 1 hour after loperamide exposure; increased BBB permeability leads to reduced locomotor activity. B . Leaky 3 dpf larvae are significantly sedated compared to 5 and 7 dpf fish, as previously reported (O’Brown, 2019). C . spock1 mutants show significantly reduced activity compared to wild-type (WT) controls at 7 dpf using FishNAP. D . Workflow schematic for assessing BBB permeability in cldn5a crispant fish. E–H . Tracer leakage assays using 1 kDa NHS ester (E, turquoise) and 10 kDa Dextran (F, gold) reveal significantly increased 1 kDa NHS tracer permeability outside of the kdrl:mCherry + vasculature (magenta) in cldn5a crispants vs. uninjected controls (G), but no significant change in 10 kDa Dextran permeability (H). I . FishNAP also detects increased BBB permeability in cldn5a crispants relative to uninjected controls. The slowest fish (red outlines) show greater than 95% cldn5a knockdown. ** p<0.01, *** p<0.001, **** p<0.0001 by one-way ANOVA (B and C) and Welches t-test (G-I). Scale bar represents 50 µm.
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    Image Search Results


    A . Schematic of the FishNAP assay, with up to seven larvae per well. Overall activity is monitored using Daniovision for 1 hour after loperamide exposure; increased BBB permeability leads to reduced locomotor activity. B . Leaky 3 dpf larvae are significantly sedated compared to 5 and 7 dpf fish, as previously reported (O’Brown, 2019). C . spock1 mutants show significantly reduced activity compared to wild-type (WT) controls at 7 dpf using FishNAP. D . Workflow schematic for assessing BBB permeability in cldn5a crispant fish. E–H . Tracer leakage assays using 1 kDa NHS ester (E, turquoise) and 10 kDa Dextran (F, gold) reveal significantly increased 1 kDa NHS tracer permeability outside of the kdrl:mCherry + vasculature (magenta) in cldn5a crispants vs. uninjected controls (G), but no significant change in 10 kDa Dextran permeability (H). I . FishNAP also detects increased BBB permeability in cldn5a crispants relative to uninjected controls. The slowest fish (red outlines) show greater than 95% cldn5a knockdown. ** p<0.01, *** p<0.001, **** p<0.0001 by one-way ANOVA (B and C) and Welches t-test (G-I). Scale bar represents 50 µm.

    Journal: bioRxiv

    Article Title: In vivo discovery of blood-brain barrier opening small molecules with FishNAP

    doi: 10.64898/2026.03.18.712473

    Figure Lengend Snippet: A . Schematic of the FishNAP assay, with up to seven larvae per well. Overall activity is monitored using Daniovision for 1 hour after loperamide exposure; increased BBB permeability leads to reduced locomotor activity. B . Leaky 3 dpf larvae are significantly sedated compared to 5 and 7 dpf fish, as previously reported (O’Brown, 2019). C . spock1 mutants show significantly reduced activity compared to wild-type (WT) controls at 7 dpf using FishNAP. D . Workflow schematic for assessing BBB permeability in cldn5a crispant fish. E–H . Tracer leakage assays using 1 kDa NHS ester (E, turquoise) and 10 kDa Dextran (F, gold) reveal significantly increased 1 kDa NHS tracer permeability outside of the kdrl:mCherry + vasculature (magenta) in cldn5a crispants vs. uninjected controls (G), but no significant change in 10 kDa Dextran permeability (H). I . FishNAP also detects increased BBB permeability in cldn5a crispants relative to uninjected controls. The slowest fish (red outlines) show greater than 95% cldn5a knockdown. ** p<0.01, *** p<0.001, **** p<0.0001 by one-way ANOVA (B and C) and Welches t-test (G-I). Scale bar represents 50 µm.

    Article Snippet: The plate was then placed in a Daniovision recording chamber (Noldus).

    Techniques: Activity Assay, Permeability, Knockdown